Information Technology Reference
In-Depth Information
Response to Autoinducer Messaging
2500
2000
pRCV-3 + pUC19
pRCV3 + pSND-1
pRCV-3
pRCV-3 + pRW-LPR-2
pRCV-3 + pTK-1 AI
1500
1000
500
0
0:00
0:30
1:00
1:30
2:00
Time (hrs)
Figure 7.16
Verification of communication constructs.
2500
2000
pRCV-3 + pUC19
pRCV3 + pSND-1
pRCV-3
pRCV-3 + pRW-LPR-2
pRCV-3 + pTK-1 AI
1500
1000
500
0
0:00
0:30
1:00
1:30
2:00
Time (hrs)
Figure 7.17
Time-series response of receivers to signal. The negative controls
(pRCV-3 and pRCV-3) show no increase in fluorescence over time. The positive con-
trol with constitutive expression of GFP(LVA) (pRW-LPR-2) shows a high level of
fluorescence. The mixed culture of senders and receivers (pRCV-3
+
pSND-1) shows
the increase in fluorescence over time due to the cell-to-cell communications. Finally,
a highly concentrated extract of VAI mixed with the receivers (pRCV-3
+
pTK-1 AI)
shows a rapid increase in fluorescence.
