Information Technology Reference
In-Depth Information
1,000.00
100.00
pINV-107-mut4/pINV-112-R3
pINV-107-mut5/pINV-112-R3
pINV-107-mut6/pINV-112-R3
10.00
1.00
0.1
1.0
10.0
100.0
1,000.0
IPTG (uM)
Figure 7.11
The effect of
λ
P(R
−
O
12
)
O
R
1 operator mutations on the behavior of the
cI
/
λ
P(R
−
O
12
)
circuit.
to convert a nonfunctional circuit with mismatched gates into a circuit that
achieves the correct response (Figure 7.2). Note that in choosing genetic vari-
ations, the specific RBS modifications described earlier can be applied to any
inverter and are independent from the specific genetic candidate. The operator
mutations in this subsection are specific to the
λ
P(R)
and
cI
and cannot be
generally applied to other operators. Accordingly, there are several strategies
to optimizing a new component. First, one can test modifications that are ap-
plicable to any component. Second, one can study the particular component
by reading the literature and performing laboratory experiments and choosing
mutations based on the understanding of the specific biochemistry of the el-
ement. Third, one can perform large-scale, random mutations on the element
and screen for mutants that have the desired behavior.
The device physics measurements in this section facilitate biocircuit design
because they enable prediction of the behavior of complex circuits using the
characteristics of simple components. With the appropriate tools, the engineer
of biocircuits can begin to design and produce large-scale circuits. In under-
standing the device physics of cellular gates, great emphasis must be placed
on considering the special substrate properties, such as signal fluctuations and
matching kinetic characteristics. The experimental results reported here repre-
sent an important effort to assemble a library of simple standardized biological
components with the proper device physics that can be combined in predictable
ways to engineer novel cell behaviors.
CELL-CELL COMMUNICATIONS
In this section, we describe engineered cell-cell communications using chemi-
cal diffusion. Communication between cells is obviously essential to any kind of
