Cellular Computation and Communication Using Engineered Genetic Regulatory Networks - Cellular Computing - page 125

aTc

Lambda P(R-O12)
RBSII

P3 P

ECFP

Kan(r)

tetR

AP r

T0 Term

pINV-206
4982 bp

pINV-203
3644 bp

lacI

p15A ori

RBSII

T1 Term

P(LtetO-1)

ColE1 ORI

P(LAC)

RBSII

EYFP

Figure 7.5 Genetic circuit to measure the transfer curve of the lacI/p(lac) inverter.

1,400

1,200

1,000

800

600

400

200

30 ng/ml
aT c

3 ng/ml
aT c

0

1 10 100 1,000

Fluorescence

(a) Digital computation noise margins (b) Gain/signal restoration

Figure 7.6 Transfer curve gain and noise margins for the lacI/p(lac) circuit.