Biomedical Engineering Reference
In-Depth Information
Table 1.1 Target
functional groups
for biosensor
immobilization of proteins.
Functional group
Amino acid
S
d
n
4
t
3
n
g
|
1
H
Cysteine
Thiol
N
H
Lysine (side chain)
H
Amine
O
H
Tyrosine
Phenol
d
n
3
.
N
N
Histidine
H
Imidazole
Tryptophan
N
H
Indole
H
N
H
Arginine
N
N
H
H
Guanidine
O
Glutamic acid, aspartic acid
H
O
Carboxylic acid
Table 1.2 Electrochemical techniques available for application in biosensor
technology.
Technique
Methodology
Potentiometry
Measures electrochemical potential at current
¼
0
Voltammetry
Measures current at applied potential; controlled concentration of
electroactive species
Amperometry
Measures current versus concentration; potential is controlled
Coulometry
Measures charge over time with controlled potential
Conductometry
Measures conductance (
¼
1/resistance) at controlled concentration
