Imaging fluorescent protein fusions in live bacteria - Methods in Microbiology - page 109

Table 4.1 Plasmids used for the construction of fluorescent protein fusions in a
variety of microbial species

Plasmid Description References

pKD46 a

l Red recombinase expression Datsenko and

Wanner (2000)

pCP20 a FLP-recombinase expression Datsenko and

Wanner (2000)

pKD3 a Contains chloramphenicol resistance cassette

flanked by FLP sequences

Datsenko and
Wanner (2000)

pKD4 a Contains kanamycin resistance cassette flanked

by FLP sequences

Datsenko and
Wanner (2000)

pSG1164 b,c

C-terminal GFPmut1 fusion for single-crossover
integration with P xyl driving downstream genes

Lewis and Marston
(1999)

pSG1154 b

C-terminal GFPmut1 fusion for double-crossover
integration at amyE driven by P xyl

Lewis and Marston
(1999)

pSG1186 b,c

C-terminal CFP fusion for single-crossover
integration

Feucht and Lewis
(2001)

pSG1187 b,c C-terminal YFP fusion for single-crossover

integration

Feucht and Lewis
(2001)

pSG1729 b

N-terminal GFPmut1 fusion for double-crossover
integration at amyE driven by P xyl

Lewis and Marston
(1999)

pSG1190 b N-terminal CFP fusion for double-crossover

integration at amyE driven by P xyl

Feucht and Lewis
(2001)

pSG1191 b N-terminal YFP fusion for double-crossover

integration at amyE driven by P xyl

Feucht and Lewis
(2001)

pSG1192 b C-terminal CFP fusion for double-crossover

integration at amyE driven by P xyl

Feucht and Lewis
(2001)

pSG1193 b

C-terminal YFP fusion for double-crossover
integration at amyE driven by P xyl

Feucht and Lewis
(2001)

pYG1 b

C-terminal GFPmut3 fusion for single-crossover
integration with P xyl driving downstream genes

Doherty et al. (2010)

pNG621 b,c C-terminal mCherry fusion for single-crossover

integration with P xyl driving downstream genes

Doherty et al. (2010)

pLOW-
GFP d

C-terminal GFPmut1 fusion. Low copy, non-
integrative

Liew et al. (2011)

pLau53 a C-terminal EYFP and ECFP fusion to tetR and lacI,

respectively. Tandem expression driven by P ara

Lau et al. (2003)

Primers l Red Template construction

GFP F TGGCGCGCTCTAGAAATGCGTAAAG Doherty et al. (2010)

GFP R e

CCCCTC GGTACCT TATTTGTATAG Doherty et al. (2010)

Cat F e AAAAAA GGTACC GTGTAGGCTGGAGCTGCTTC Doherty et al. (2010)

Cat R AAAAAATCATGAATGGGAATTAGCCATGGTCC Doherty et al. (2010)

a For use in E. coli.
b For use in Bacillus spp.
c For use in Acinetobacter spp.
d For use in S. aureus.
e Acc651 restriction sites used for joining gfpmut3 to chloramphenicol resistance gene are underlined.