Biomedical Engineering Reference
In-Depth Information
negative control plate easily outnumber the colonies on the
cloning plate, we have found that the majority of the colonies
on the cloning plate to be correct. Thus these should not be
disposed without being examined.
Acknowledgement
This work was supported by a strategic PhD stipend from the
Faculty of LIFE Sciences.
References
1. Mertz JE, Davis RW (1972) Cleavage of DNA
by R1 restriction endonuclease generates
cohesive ends. Proc Natl Acad Sci USA
69:3370-3374
2. Gellert M (1967) Formation of covalent cir-
cles of lambda DNA by
E. coli
extracts. Proc
Natl Acad Sci USA 57:148-155
3. Weiss B, Richardson CC (1967) Enzymatic
breakage and joining of deoxyribonucleic acid,
I. Repair of single-strand breaks in DNA by an
enzyme system from
Escherichia coli
infected
with T4 bacteriophage. Proc Natl Acad Sci
USA 57:1021-1028
4. Smith HO, Welcox KW (1970) A restriction
enzyme from
Hemophilus infl uenzae
: I.
Purifi cation and general properties. J Mol Biol
51:379-391
5. Mullis K, Faloona F, Scharf S et al (1986)
Specifi c enzymatic amplifi cation of DNA in
vitro: the polymerase chain reaction. Cold
Spring Harb Symp Quant Biol L1:263-273
6. Mullis KB, Faloona FA (1987) Specifi c synthe-
sis of DNA in vitro via a polymerase-catalyzed
chain
11. Yon J, Fried M (1989) Precise gene fusion by
PCR. Nucleic Acids Res 17:4895
12. Aslanidis C, De Jong PJ (1990) Ligation-
independent cloning of PCR products (LIC-
PCR). Nucleic Acids Res 18:6069-6074
13. Jones D, Sakamoto K, Vorce R et al (1990)
DMA mutagenesis and recombination. Nature
344:793-794
14. Shuldiner AR, Scott LA, Roth J (1990) PCR-
induced (ligase-free) subcloning: a rapid reli-
able method to subclone polymerase chain
reaction (PCR) products. Nucleic Acids Res
18:1920
15. Nisson PE, Rashtchian A, Watkins PC (1991)
Rapid and effi cient cloning of Alu-PCR prod-
ucts using uracil DNA glycosylase. Genome
Res 1:120-123
16. Smith C, Day P, Walker MR (1993) Generation
of cohesive ends on PCR products by UDG-
mediated excision of dU, and application for
cloning into restriction digest-linearized vec-
tors. Genome Res 2:328-332
17. Yang YS, Watson WJ, Tucker PW et al (1993)
Construction of recombinant DNA by exonu-
clease
reaction.
Methods
Enzymol
155:335-350
7. Scharf SJ, Horn GT, Erlich HA (1986) Direct
cloning and sequence analysis of enzymatically
amplifi ed
recession.
Nucleic
Acids
Res
21:1889-1893
18. Gibson DG, Young L, Chuang RY et al (2009)
Enzymatic assembly of DNA molecules up to
several hundred kilobases. Nat Methods
6:343-345
19. Evans DH, Willer DH, Yao XD (2003) DNA
joining method. US Patent US/2003/0162265
A1
20. Li MZ, Elledge SJ (2007) Harnessing homol-
ogous recombination in vitro to generate
recombinant DNA via SLIC. Nat Methods
4:251-256
21. Bitinaite J, Rubino M, Varma KH et al (2007)
USER™ friendly DNA engineering and clon-
ing method by uracil excision. Nucleic Acids
Res 35:1992-2002
genomic
sequences.
Science
233:1076-1078
8. Ho SN, Hunt HD, Horton RM et al (1989)
Site-directed mutagenesis by overlap extension
using the polymerase chain reaction. Gene
77:51-59
9. Horton RM, Hunt HD, Ho SN et al (1989)
Engineering hybrid genes without the use of
restriction enzymes: gene splicing by overlap
extension. Gene 77:61-68
10. Vallette F, Mege E, Reiss A et al (1989)
Construction of mutant and chimeric genes
using the polymerase chain reaction. Nucleic
Acids Res 17:723-733
