Biomedical Engineering Reference
In-Depth Information
Fig.
2
Screenshot of the Primer Help tool for USER (
http://www.cbs.dtu.dk/services/PHUSER/
)
. Three important
areas are
highlighted
: (A) where you insert your sequence(s); (B) where you specify the maximum desired
length of PCR products; and (C) where you select the target USER cassette
3. Decide the maximum PCR product length using the drop-
down menu marked with B (Fig.
2
) (
see
Note 4
).
4. Choose the USER cassette corresponding to one of your plas-
mid, i.e., having the same directional bases, and restriction-
and nicking-sites by using the drop-down menu identifi ed by
C (Fig.
2
) (
see
Notes 5
and
6
). Press “submit query.” The
program returns the primers needed for cloning fusion into the
