Biomedical Engineering Reference
In-Depth Information
Fig.
8
An example of in silico PCR result
type, allowing users to see the results immediately on screen. They
can also calculate the volume of solvent required to attain a specific
concentration from the known mass (mg), OD, or moles of
oligonucleotide.
All primers are analyzed for intra- and inter-primer interactions
regarding formation of dimers. Primer(s) can efficiently hybridize
using the 5′ end or the middle of the oligonucleotides. Even
though such interactions are not efficiently extended by DNA
polymerase, their formation reduces the effective primer concen-
tration available for binding to the targets and their presence can
strongly inhibit PCR because double-stranded DNA at high con-
centrations is a strong inhibitor of DNA polymerase (Fig.
9
).
