Biomedical Engineering Reference
In-Depth Information
11. We provide three SLiCE reaction systems (
see
Table
1
).
Standard cloning is for general cloning of one or multiple
DNA fragments into vectors [
4
]. Rush cloning is an alternative
approach mainly for simple cloning. BAC cloning is for direc-
tional subcloning of DNA fragments from BAC vectors [
4
].
12. Standard transformation methods such as electroporation and
chemical transformation are all compatible with SLiCE cloning.
For cloning of large or complex DNA fragments, electroporation
is recommended (yielding 10-100-fold higher transformation
effi ciencies than that of chemical transformation).
Acknowledgement
This work was supported by National Institute of Health
[1R01CA76329 to W.E., 1R01CA93484 to W.E].
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