Application of In-Fusion Cloning for the Parallel Construction of E. coli Expression Vectors - DNA Cloning and Assembly Methods - page 217

Vector Tag Signal sequence Pathway

Origin
(copy no.) Resistance

Vector
description sites

Forward
extension

Reverse
extension

pOPINDsbA SS-POI-C-HIS E. coli DsbA SRP pUC (50

to >100)

Amp T7lacO, CMV

enhancer and
bb-actin
promoter, p10
promoter/
lef-2 and 1629
baculo
elements

KpnI PmeI DsbAss_InfFwd

TTTAGCGC
ATCGGCG

KH6-pTriExInfrev

MKKIWLALAG
LVLAFSASA

GTGATGGT
GATGTTT

pOPINTolB SS-POI-C-HIS E. coli TolB SRP pUC (50

to >100)

Amp T7lacO, CMV

enhancer and
bb-actin
promoter, p10
promoter/
lef-2 and 1629
baculo
elements

KpnI PmeI TolBss_InfFwd KH6-pTriExInfrev

MKQALRVAFGFLI
LWASVLHA

CATCAGTTC
TGCATGCT

GTGATGGT
GATGTTT

All vectors are linearized with KpnI and PmeI and use the same 3 ′ extension as pOPINE (Table 2 ; pOPINE can be used to make a vector with a native leader sequence if appropriate) but have

signal sequence-specifi c 5 extensions. The secretory pathway targeted is indicated in the table.

POI protein of interest, C-His C-terminal His 6 tag, SS signal sequence.

′