Application of In-Fusion Cloning for the Parallel Construction of E. coli Expression Vectors - DNA Cloning and Assembly Methods - page 210

Table 2
Vectors designed for fusion at the C-terminus of proteins (pOPINE family)

Vector
linearization
sites

Origin
(copy no.)

Vector
backbone Vector description

Reverse
extension Vector Tag Resistance Forward extension

pOPINE POI-C-HIS pUC (50

to >100)

Amp pTriEX2 T7lacO, CMV

enhancer and
bb-actin promoter,
p10 promoter/
lef-2 and 1629
baculo elements

NcoI PmeI Kozak(pET/TriEx)

Inffwd

KH6-pTriExInfrev

AGGAGATAT
ACCATG

GTGATGGTGA
TGTTT

pOPINE-3C-
eGFP

POI-3C-eGFP-
C-HIS

pUC (50
to >100)

Amp pTriEX2 T7lacO, CMV

enhancer and
bb-actin promoter,
p10 promoter/
lef-2 and 1629
baculo elements

NcoI PmeI Kozak(pET/TriEx)

Inffwd

3CFusionRev

AGGAGATAT
ACCATG

CAGAACTTCC
AGTTT

pOPINE-3C-
HALO7

POI-3C-Halo7-
C-HIS

pUC (50
to >100)

Amp pTriEX2 T7lacO, CMV

enhancer and
bb-actin promoter,
p10 promoter/
lef-2 and 1629
baculo elements

NcoI PmeI Kozak(pET/TriEx)

Inffwd

3CFusionRev

AGGAGATAT
ACCATG

CAGAACTTCC
AGTTT