Biomedical Engineering Reference
In-Depth Information
Fig.
3
Multipartite assembly of a transcriptional unit in a level
α
plasmid and Binary combination of two units in
a level
plasmid. (
a
) Combination GBparts (pE:35s:YFN, pE_FUL and pE_T35s) to build the TU pEGB_35s:
YFN::FUL:T35s in the vector pDGBA12C. (
b
) Combination GBparts (pE:35s:YFC, pE_SOC1 and pE_T35s) to build
the TU pEGB_35s:YFC::SOC1:T35s in the vector pDGBC12B. (
c
) Assembly of the complementary TUs pEGB_
A- 35s:YFN::FUL:T35s-C and pEGB_C-35s:YFC::SOC1:T35s-B in the destination vector pDGB1AB3. (
d
) Digestion
of correct clones of the multipartite assemblies pEGB_A-35s:YFN::FUL:T35s-C (
Lane 2
: BglII;
Lane 23
: PvuI + NcoI)
and pEGB_C-35s:YFC::SOC1:T35s-B (
Lane 5
: BglII;
Lane 6
: HindIII) (E) BanI (
Lane 2
) and BglI (
Lane 3
) digestion of
a correct clone of the assembly of the two TUs pEGB_1-35s:YFN::FUL:T35s-35s:YFC::SOC1:T35s-3
Ω
