Contractile Vacuole Complex—Its Expanding Protein Inventory - International Review of Cell and Molecular Biology - page 381

Table 9.3 Effects of gene silencing on the CVC of Paramecium
Component Effect Reference

Actin
Actin 9 10 times prolonged contraction

cycles

Sehring et al.
(2007a)

Other actin isoforms No effect Sehring et al.

(2007a)

SNARE-specific chaperone

NSF Reduced smooth and decorated

spongiome formation (EM), prolon-
gated CVC cycles

Sch¨nemann
et al. (2013)

SNAREs
Syb2 Slowed pulsation of CV Sch¨nemann

et al. (2013) CV swelling with high [Ca 2 þ ] o

Deformed cells with swelling of radial
canals and prolongated CVC cycles

Schilde et al.
(2006)

Syb6 CV swelling and reduced pulsation

activity

Sch¨nemann
et al. (2013)

CV swelling with high [Ca 2 þ ] o

Syx2 CV swelling and reduced pulsation

activity

Sch ¨ nemann
et al. (2013)

CV swelling with high [Ca 2 þ ] o

Syx6 Aberrant form and position

of new CVCs

Sch¨nemann
et al. (2013)

No effect with high [Ca 2 þ ] o , yet
slowly a decreasing viability

Syx14 Sch¨nemann

et al. (2013)

No effect with high [Ca 2 þ ] o , yet
immediate b loss of viability

Syx15

Sch¨nemann
et al. (2013)

SNAP-25-LP No clear effect Schilde et al.

(2008)

H þ -ATPase
c2 or c4 or c6 SUs or
combinations of c-SUs

5-10 times prolonged contraction
cycles

Wassmer
et al. (2005)
Continued