Contractile Vacuole Complex—Its Expanding Protein Inventory - International Review of Cell and Molecular Biology - page 374

Table 9.2 Components of contractile vacuole complex reported from
other protists — cont'd
Organisms Component Method Reference

H þ -PPase
(pyrophosphatase)

Trypanosoma Montalvetti et al. (2004)

H þ -ATPase SU B Proteomics h

Ulrich et al. (2011)

Calmodulin Proteomics Ulrich et al. (2011)

TcAQP (aquaporin) Genomics Montalvetti et al. (2004)

Rab11 Proteomics Ulrich et al. (2011)

Rab32 Proteomics Ulrich et al. (2011)

AP-180 Proteomics Ulrich et al. (2011)

VAMP1 (v-SNARE) Proteomics Ulrich et al. (2011)

Tc SNARE2.1 Proteomics Ulrich et al. (2011)

Tc SNARE2.2 Proteomics Ulrich et al. (2011)

Disgorgin Proteomics Ulrich et al. (2011)

Myosin Proteomics Ulrich et al. (2011)

Clathrin heavy chain Proteomics Ulrich et al. (2011)

InsP 3 R/RyR-related
protein

Proteomics Ulrich et al. (2011)

a AP-2, adaptor protein 2 (for clathrin coat assembly).
b pABs, polyclonal antibodies.
c mABs, monoclonal antibodies.
d HA, hemagglutinin.
e NIMA-related kinases are Ser/Thr kinases implicated in cell-cycle control.
f Some of the components listed have been shown to associate with the CVC of Dictyostelium only tran-
siently, notably Disgorgin, LvsA, Rab8A, and Drainin ( Du et al., 2008 ).
g Red fluorescent protein.
h Assignment to the CVC based on enriched fractions.

membrane-to-membrane interactions also outside the pore and the CV/
ampullae connections. Their occurrence reflects the fusion capacity
in vitro of vesicles derived from the Paramecium CVC ( Sugino et al.,
2005 ). In vivo fusion may serve for permanently ongoing biosynthesis by ves-
icle delivery. This also makes CVC-resident Pt SNAREs relevant for
maintaining Ca 2 þ homeostasis ( Sch ¨ nemann et al., 2013 ). Moreover, they
may account for reversible fusion/fission processes within the spongiome for
regulating membrane area during swelling and collapse of the CV and the
radial arms during diastole/systole cycles ( Sch¨nemann et al., 2013 ). In