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Table 9.1 Components of contractile vacuole complex localized to the CVC of
Paramecium a
Components
Method
Reference
SNARE-specific chaperone
NSF
Immuno-LM
Kissmehl et al. (2002)
SNAREs
Syb2
GFP, immuno-LM Schilde et al. (2006)
GFP/immuno-EM
Syb6
Immuno-LM/-EM Sch¨nemann et al. (2013)
Syb9
Immuno-LM/-EM Sch ¨ nemann et al. (2013)
Schilde et al. (2010) b
Syb10
Immuno-LM
Syx2
GFP
Kissmehl et al. (2007)
Syx14
GFP
Kissmehl et al. (2007)
Immuno-LM/EM
Sch¨nemann et al. (2013)
Syx15
GFP
Kissmehl et al. (2007)
SNAP-25-LP
GFP, immuno-LM Schilde et al. (2008)
H þ -ATPase
c1, c4, c5 SUs
GFP
Wassmer et al. (2005, 2006, 2009)
F2
GFP
Wassmer et al. (2005, 2006, 2009)
a2
Immuno-LM
Fok et al. (1995)
GFP
Wassmer et al. (2006)
g -Tubulin
Immuno-LM
Klotz et al. (2003)
Acetylated tubulin
Immuno-LM
Callen et al. (1994)
Calmodulin
Immuno-LM/EM
Momayezi et al. (1986)
Fok et al. (2008)
Ca -release channels
IP 3 R
Immuno-LM
Ladenburger et al. (2006)
Scaffolding proteins
Stomatin1
Immuno-LM
Reuter et al. (2013)
Stomatin4
Immuno-LM
Reuter et al. (2013)
a
For subcellular localization, see text.
b Only weak labeling.
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