Contractile Vacuole Complex—Its Expanding Protein Inventory - International Review of Cell and Molecular Biology - page 368

Figure 9.1 Examples of several CVC components of P. tetraurelia visualized by fluores-
cence microscopy. (A) Syb2-GFP stains the CV and radial arms with adjacent smooth
spongiome (confirmed by high-resolution immuno-EM). (B) NSF localized by anti-NSF
antibodies under precautions allowing for the retention of the antigen at the sites of
NSF activity. Note staining of CV and radial arms with adjacent smooth spongiome
(evident from comparison with (A)) and hot spots at the CV/radial arms junctions
(arrowheads). (C) H þ -ATPase visualized by the F-subunit (of the catalytic V1 part) as a
GFP-fusion protein reveals its localization to the decorated spongiome (confirmed by
immuno-EM). cv, contractile vacuole; ds, decorated spongiome; ra, radial arm (with
decorated spongiome attached). Bars ¼ 10 mm. (A) From Schilde et al. (2006) , (B) from

Kissmehl et al. (2002) , and (C) from Wassmer et al. (2005) .