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Figure 7.8 Effect denervation on rat EDL muscle as quantified in an automated fashion
by CyteSeer ® 's Skeletal Muscle Algorithm. (A) An image from a control muscle labeled
for laminin (green) is shown. (B) Fiber outline mask and fiber ID#s overlaid on the gray-
scale image from (A). (C) An example image from a denervated muscle. (D) Fiber outline
mask and fiber ID#s overlaid on the gray-scale image from (C). (E) The effect of dener-
vation (Den) on CSA. Each bar represents the meanSD for n¼6 images/condition,
with
80
-
135 fibers per control image and
193
-
282 fibers per denervated image.
*** p <
0.00001 by
t
-test.
Figure 7.9 Analysis of NCAM expression in mouse skeletal muscle. (A) An image of skel-
etal muscle from an SOD1-null mouse labeled for laminin (green) and NCAM (red) is
shown. Also shown is the fiber mask (which delineates the inside of each fiber). (B)
The image displayed for NCAM only. The arrow identifies an example fiber that is pos-
itive for NCAM. (C) Quantification of fiber size as a function of NCAM expression. Bars are
meanSEM for n¼78 fibers that were low in NCAM expression (NCAM) and
n¼44 fibers that were positive for NCAM (NCAMþ). *** p < 0.001 by t-test.
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