Biology Reference
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Figure 7.5 Identification of muscle fiber outlines in muscle sections from different spe-
cies by CyteSeer ® . Sections stained for laminin are shown for mouse (A), swine (B), and
monkey (C). The corresponding fiber outlines derived from these images by the Skeletal
Muscle Algorithm are shown in (D), (E), and (F).
6.1.2 Quantification of fiber metabolic type
Once an algorithm for identifying the muscle fiber outlines has been
established, the fiber outline mask can then be projected onto images
obtained from other fluorescence channels, each of which can potentially
represent a fiber-type specific biomarker. To test the effectiveness of this
procedure, muscle samples from monkey were labeled for laminin plus
slow-type myosin and swine muscle samples were labeled for laminin and
for myosin IIA. The samples were imaged and the images quantified for pos-
itive fibers by Dr. Kostrominova by the manual method and by the
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