New Insights into the Role of BubR1 in Mitosis and Beyond - International Review of Cell and Molecular Biology - page 219

CHAPTER SIX

New Insights into the Role
of BubR1 in Mitosis and Beyond

Roger E. Karess *

,1
* Institut Jacques Monod, UMR 7592 CNRS, Universit´ Paris Diderot-Paris 7, Paris, France
† Developmental Biology Lab, UMR 7622 CNRS, University of Paris 6, Paris, France
1 Corresponding authors: e-mail address: [e-mail]; rahmani.zohra@ijm.
univ-paris-diderot.fr

,1 , Katja Wassmann † , Zohra Rahmani *

Contents
1.

Introduction 224

2. Chromosome Segregation and Cell Cycle Machinery 225

2.1 Mitosis, SAC, and kinetochores 225

2.2 Chromosome attachment and error correction 226

2.3 Kinetochore assembly 227

3. BubR1 Structure 228

3.1 N-terminal domain 229

3.2 Kinase domain 230

3.3 KARD domain 230

3.4 Posttranslational modifications and interacting proteins 230

3.5 BubR1, Bub1, and Mad3: Evolutionary considerations 235

4. BubR1 during Mitosis 235

4.1 Recruitment to kinetochores 235

4.2 BubR1 and assembly of the MCC 240

4.3 BubR1 and K-MT attachment 244

4.4 The mitotic timer 248

4.5 BubR1 acetylation and sumoylation 249

5. Nonmitotic Functions 250

5.1 Meiosis 250

5.2 DNA damage response 254

5.3 Centrosomes and cilia 256

6. BubR1 and Disease 257

6.1 Mosaic variegated aneuploidy 257

6.2 Senescence and cell differentiation 258

6.3 BubR1 and cancer 259

7. Concluding Remarks 262

Acknowledgments 263

References 263