Regulation of Cell Polarity and RNA Localization in Vertebrate Oocytes - International Review of Cell and Molecular Biology - page 145

Table 4.2 Vegetally localized mRNAs in Xenopus—cont'd
Gene
symbol References

dead end homolog 1 dnd1 Weidinger et al. (2003)

RNA-binding protein with multiple
splicing ( hermes)

rbpms Zearfoss et al. (2004)

glutamate receptor-interacting protein 2
( grip2)

grip2 Kaneshiro et al. (2007)

and Tarbashevich et al. (2007)

low molecular weight neuronal
intermediate filament

nif Claussen et al. (2004)

vegetally localized 1 velo1 Claussen and Pieler (2004)

Strategies for identifying localized RNAs in the oocyte

Vegetal cortex isolation/Affymetrix
microarray

Cuykendall and Houston (2010)

Differential hybridization to cDNA
arrays

Kataoka et al. (2005)

Computational analysis of 3 0 -UTRs

Betley et al. (2002)

Differential display PCR Hudson et al. (1996)

Differential cDNA library screening Zhang and King (1996)

Differential cDNA library screening Rebagliati et al. (1985)

RNAs with previously described vegetal localization in Xenopus oocytes. References cite the primary
description of the localization pattern. Intermediate pathway RNAs are defined as having mainly a late
localization pattern, but with additional localization to the mitochondrial cloud and to the germ plasm in
embryos. Other uncharacterized localized transcripts can be found in works referenced at the bottom of
the table.

in movement of mitochondrial cloud components to the vegetal cortex
( Deshler et al., 1997; Kloc and Etkin, 1998 ). During stages III-IV, gdf1 trans-
locates to the vegetal cortex and remains anchored there in full-grown
oocytes ( Alarc´n and Elinson, 2001; Yisraeli et al., 1990 ). Anchoring is sat-
urable ( Yisraeli et al., 1990 ), implying discrete targets, and is thought to
depend on cortical cytokeratin filaments and cortical membranous elements
( Alarc´n and Elinson, 2001; Pondel and King, 1988 ). Gdf1 is released during
oocyte maturation and remains enriched in the vegetal hemisphere.
Cytoskeleton-disrupting drug experiments showed that the initial accu-
mulation of gdf1 in the ER-wedge is independent of the cytoskeleton,