Biomedical Engineering Reference
In-Depth Information
Fig. 3.11
Efficiency of interaction between siRNA hybridization probes and bcr/abl mRNA
hybridization samples-FRET analysis.
a
Typical fluorescent spectra of nonhybridized and hybrid-
ized QD-labeled siRNA probe and Cy5-labeled mRNA sample (ìex) 420 nm. 1: QD-siRNA-6
(mismatch). 2: QD-siRNA-6 plus Cy5-labeled mRNA. 3: QD-siRNA-5 plus Cy5-labeled mRNA.
b
FRET between QD-labeled siRNAs and Cy5-labeled target mRNA. The results on histograms
represent the mean (SD from seven-independent experiments for siRNA-1, 2, and 6, and from
six independent experiments for siRNA-3, 4, and 5.
c
Correlation between FRET data (obtained
in this study) and effect of anti-bcr/abl siRNAs on the level of target mRNA (obtained from the
