Biomedical Engineering Reference
In-Depth Information
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SCHEME 4.37
Cellular cholesterol levels in zebrafish embryos.
large number of compounds for their impacts on the formation and function of the
heart and its vessels. We expected to observe effects on several phenotypes, including
changes in the populations of cardiac and endothelial precursors, abnormal patterning
of the heart and the vasculature, and abnormal cardiac functions, including arrhyth-
mia. Using this model, we identified a novel compound, aplexone, which affects
the transcription of the HMG-CoA reductase pathway and differentially regulates
arteriovenous angiogenesis in zebrafish and human endothelial cells. Aplexone was
also effective in lowering the cellular cholesterol levels—even more efficiently than
atorvastatin (Scheme 4.37) [68].
4.5 CONCLUSIONS
We have established collections of compound libraries, based on phosphine catal-
ysis of allenes and acetylenes, as common starting points for chemical biological
