Biomedical Engineering Reference
In-Depth Information
Table 1 Pure and mixed cultures for hydrogen production
Microorganism
Conditions
pH
Substrate
H
2
yield
References
Pure cultures
Dark-fermentative bacteria
Clostridium beijerinckii
AM21B
Batch
6.5
Glucose
2.0 mol H
2
/mol
glucose
[
13
]
Clostridium beijerinckii
Fanp3
Batch
6.47-6.98
Glucose
2.52 mol H
2
/
mol glucose
[
14
]
Pantoea agglomerans
Batch
6.0
Glucose
2,246 mL H
2
/L
culture
[
15
]
Clostridium beijerinckii
RZF-1108
Batch
7.0
Glucose
1.97 mol H
2
/
mol glucose
[
16
]
Ethanoligenens
harbinense B49
Batch
3.9-4.2
Glucose
1.6-1.8 mol H
2
/
mol glucose
[
17
]
Ethanoligenens
harbinense YUAN-3
Batch
Glucose
2.81 mol H
2
/
mol glucose
[
18
]
Klebsiella pneumoniae
ECU-15
Batch
6.6
Glucose
2.07 mol H
2
/
mol glucose
[
19
]
Photofermentative bacteria
Rhodopseudomonas
faecalis strain RLD-53
Batch
7.0
2.84 mol H
2
/
mol acetate
[
20
]
Rhodopseudomonas
palustris CQK 01
Batch
Glucose
12.04 mmol H
2
/
300 mL
[
21
]
Mixed cultures
Sewage sludge
Continuous
6.7
Sucrose
1.7 mol H
2
/mol
glucose
[
13
]
Mixed culture
Continuous
No
Sucrose
3.47 mol H
2
/
mol sucrose
[
22
]
control
Boiling treated
anaerobic sludge
Batch
Municipal
solid
waste
134 mL H
2
/g
volatile
solids
[
23
]
involved in hydrogen production, was isolated from E. harbinense YUAN-3 and
overexpressed in Escherichia coli BL-21 [
24
]. The hydA gene consists of an
open reading frame of 1,743 bp, encoding 580 amino acids for the hydrogenase
with an estimated molecular mass of 63.18 kDa, and the purified enzyme from
the recombinant exhibits catalytic activity in vitro as well as in vivo. The acetate
kinase (ack) gene, which is responsible for the dephosphorylation of acetyl
phosphate with the concomitant production of acetate and ATP, was isolated
from E. harbinense B49 [
25
]. This gene contains an open reading frame of
1,200 bp, encoding 399 amino acids for the enzyme with an estimated molecular
mass of 43.22 kDa and an isoionic point of 5.93. The ack gene was heterolo-
gously expressed in E. coli BL21 (DE3) as an inclusion body, and the specific
activity of the refolded enzyme was 42.12 U at 25 C, with 14.36% recombinant
protein renatured. The activity of ack is closely related to acetate excretion in
dark
fermentation,
which
then
regulates
metabolism
in
hydrogen-producing
bacteria.
Search WWH ::
Custom Search