Biomedical Engineering Reference
In-Depth Information
Fig. 1 Toxicity of 3-methyl-
1-butanol for Escherichia coli
(a) and Corynebacterium
glutamicum (b) OD600
optical density at 600 nm
purification extremely energy intensive and resulting in a significant amount of
waste being discharged. Engineering host strains with improved tolerance is the
primary solution [
43
], and has been well demonstrated in butanol production with
Clostridium acetobubutylicum [
44
].
4.2 Improving Alcohol Tolerance of Microorganisms
Methods of improving the alcohol tolerance of microorganisms can be grouped
into two strategies: random approach and rational design [
44
]. Improving alcohol
tolerance by the random approach includes random mutation via evolutionary
engineering. This strategy was used in the authors' laboratory to improve the
3-methyl-1-butanol tolerance of E. coli. A twofold increase in tolerance was
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