CELL - BASED NANOCOMPOSITES AND BIOMOLECULES FOR BONE TISSUE ENGINEERING - Advanced Biomaterials: Fundamentals, Processing, and Applications - page 545

TABLE 16.1. Effects of Culture Media Supplements on Osteogenic Markers In Vitro

Cells Function References

Ref 20

Human
bone
marrow-
derived
primary
osteoblasts

1. Dexamethasone enhanced ALP activity but not BMP - 2.
2. Dexamethasone caused the downregulation of Type I
collagen.
3. Osteocalcin synthesis was dependent on Vitamin D.
Dexamethasone or BMP-2 alone had no effects on
the basal osteocalcin levels but in the presence of
Vitamin D, BMP-2 increased osteocalcin synthesis,
whilst dexamethasone suppressed the production of
osteocalcin.
4. Parathyroid hormone - induced cyclic adenosine mono
phosphate (cAMP) production was signifi cantly
increased with the treatment of dexamethasone.
5. Mineralization was enhanced in cultures containing
BMP-2 and dexamethasone elicited mineralization to a
lesser degree.
6. Dexamethasone signifi cantly increased cell
proliferation.

Ref 21

Rat bone
marrow
stromal
cells and
osteoblastic
MC3T3- E1
cells

Bone marrow stromal cells
1. Vitamin C was essential for doubling cell viability.
2. Dexamethasone and β - glycerophosphate reduced the
cell proliferation rate. On the other hand, BMP,
TGF - β, vitamin D sustained the growth rate given by
Vitamin C.
3. Vitamin C in Dulbecco ' s modifi ed Eagle (DME)
medium was vital for the rapid proliferation.

MC3T3 - E1 cells
1. The incorporation of Vitamin C, Vitamin D, TGF -

and BMP did not give rise to a signifi cant increase in
cell proliferation.
2. Dexamethasone with Vitamin C and

β

-
glycerophosphate increased the cell proliferation.
3. There was no increase of cells in the samples treated
with Vitamin C in DME medium.

β

Mouse
embryo -
derived
NIH3T3
Fibroblasts

Ref 22

1. ALP activity was induced by 1 , 25 - dihydroxyvitamin

D3 in a dose-dependent manner and enhanced in the
presence of dexamethasone.
2. Dexamethasone when used alone did not caused any
detactable ALP expression.
3. Osteocalcin and osteopontin were produced in
the presence of 1

α

α , 25 - dihydroxyvitamin D3 and

dexamethasone.
4. In the presence of

β - glycerophosphate and L -

ascorbic acid plus 1 , 25 - dihydroxyvitamin D3 and

dexamethasone, extensive mineralization was observed.

α