Biomedical Engineering Reference
In-Depth Information
Fig. 3.15
SEM micrographs
of nHA/PHBV composite
scaffold surface with culture
of Saos-2 after 7 days
The morphology of Saos-2 cells on the surface of the scaffolds specimens were
revealed by SEM analysis. After 1 day of culture, it was observed from the SEM
that the flat cells were attached on PHBV, 50/50 PHBV/PLLA and 10 % HA in
50/50 PHBV/PLLA scaffolds and they had stretched their filopodias. The cells
showed filopodia anchorage on the surface of all the scaffolds. The number of
cells was more in the 10 % HA in composite scaffold than PHBV scaffold. After
day 7 of Saos-2 cultures, SEM observations confirmed that the Saos-2 cells had
proliferated well on all types of scaffolds (Fig.
3.15
). The cells on the surface
of the scaffolds specimens appeared to be more elongated as well as very well
attached and spread. The difference in morphology seen among the cells grown on
PHBV and 10 % HA in PHBV scaffolds was not noticeable. The cells were found
anchored to the surface by discrete filopodia. It was also found that the cells are
penetrating into the scaffolds through the pores.
Acknowledgments
This work was supported by a GRF grant (HKU 7182/05E) from the
Research Grants Council of Hong Kong. N. Sultana thanks The University of Hong Kong
(HKU) for providing her with a research studentship. N. Sultana also acknowledges Prof. Min
Wang for his kind supervision. Assistance provided by technical staff in the Dept. of Mechanical
Engineering, HKU, is acknowledged.
References
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ASTM standard F 1635-04a (2004) Standard test method for in vitro degradation testing of
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International, West Conshohocken
Burkersroda FV, Schedl L, Göpferich A (2002) Why degradable polymers undergo surface ero-
sion or bulk erosion. Biomaterials 23(21):4221-4231
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