Biomedical Engineering Reference
In-Depth Information
method. of. seeding. an. embryo. with. luorescent. microspheres. that. are. used. as. “artiicial. guide. stars.”.
he. experiment. for.
Drosophila
. embryo. tissue. samples. for. the. adaptive. optical. wide-ield. microscope.
has.demonstrated.a.Strehl-ratio.improvement.as.high.as.10×.when.imaging.through.100.μm.of.tissue..
For. the. confocal. microscope,. the. wavefront. measurements. were. taken. using. luorescent. microsphere.
guide-stars.and.also.using.luorescent.protein.guide-stars.that.are.incorporated.in.the.cellular.structure.
of.the.tissue.being.studied..he.experiment.for.the.ixed.mouse-brain.tissue.sample.for.the.AO.confocal.
microscope.demonstrated.a.4.3×.improvement.in.the.Strehl.ratio.and.2.4×.increase.in.intensity.in.the.
inal.image.ater.wavefront.error.correction..hese.AO.microscopes.with.direct.wavefront.sensing.can.
efectively.measure.and.correct.aberrations.at.a.high.speed,.which.is.important.for.live.in.vivo.imaging.
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