Biomedical Engineering Reference
In-Depth Information
(a)
(b)
40
μ
m
60
μ
m
80
μ
m
(c)
(d)
60
μ
m
95
μ
m
1
2.5
Without AO
0.8
2
With AO
0.6
1.5
0.4
1
Without AO
With AO
0.2
0.5
0
0
0
20
40 60
Depth (
μ
m)
(e)
80
100
0
20
40 60
Depth (
μ
m)
80
100
(f )
FIGuRE 17.27
(
See color insert.
).Comparison.of.penetration.depths.between.without.and.with.correction.for.
imaging.of.cycle.13.ly.embryos.with.EGFP-Cnn.label..(a)-(b).he.maximum.intensity.projection.of.the.scan.series.
from.the.top.surface.to.100.μm.with.and.without.adaptive.optics..(c)-(d).he.three-dimensional.reconstructions.
with.and.without.AO..(e)-(f).he.Strehl.ratio.and.PSF.size.change.for.diferent.depth..he.black.and.gray.lines.indi-
cate.without.and.with.AO,.respectively..he.scale.bar.is.10.μm.