Biomedical Engineering Reference
In-Depth Information
λ
0.04
λ
0.5
0.4
0.3
0.2
0.1
0
-
0.1
-
0.2
-
0.3
-
0.4
-
0.5
1
0.5
0.02
1
0.5
0
0
-
0.02
0
-
0.5
-
1
-
0.5
-
0.04
10
-
1
10
-
0.06
10
10
5
5
5
5
0
0
0
(a)
0
(b)
200
200
150
150
100
100
50
50
0
0
(c)
(d)
FIGuRE 17.21
Wavefront.measurements.from.a.luorescent.microsphere.guide-star.through.100-μm-thick.brain.
tissue..Wavefront.error.before.correction.(a).and.ater.correction.(b)..he.RMS.errors.for.(a).and.(b).are.0.24λ.and.
0.028λ.(λ.=.633.nm),.respectively..Images.of.the.microsphere.guide-star.before.correction.(c).and.ater.correction.
(d)..he.scale.bar.is.1.μm.
TABlE 17.3
Statistical.Properties.of.the.Strehl.Ratio.for.Brain.Tissues
Strehl.ratio
Before.correction
Ater.correction
No.
hickness.(μm)
Mean
Std.Dev
Mean
Std.Dev
1
15
0.52
0.025
0.99
0.003
2
50
0.27
0.027
0.96
0.021
3
100
0.05
0.017
0.92
0.034
intensity.increases.by.240%..he.wavefront.error.for.the.crimson.guide-star.before.correction.is.shown.
in.Figure 17.21a,.which.sufers.a.large.amount.of.spherical.aberration.(Tao.et.al..2011)..he.RMS.error.
is.0.24λ..Ater.correction,.the.RMS.wavefront.error.decreased.to.0.028λ.as.shown.in.Figure.17.21b..he.
images.of.the.microsphere.before.and.ater.correction.are.shown.in.Figure.17.21c.and.d..he.Strehl.ratio.
was.measured.using.the.method.described.in.Azucena.et.al..(2010).at.10.diferent.positions.on.each.sam-
ple.(Table.17.3)..he.improvement.in.the.Strehl.ratio.was.approximately.4.3×.for.a.100-μm-thick.sample.
17.3.2.3 comparison between a commercial confocal
Microscope and the Ao confocal Microscope
To.compare.our.system's.performance.with.a.standard.commercial.confocal.system.(Leica.TCS.SP5.II),.
the.resolution.was.investigated.for.a.50-μm-thick.brain.tissue.sample.on.both.systems.using.objective.
