Biomedical Engineering Reference
In-Depth Information
the.out-of-focus.luorescence.signals.were.suppressed..To.ind.the.improvement.trend.according.to.the.
imaging.depth,.mean.photon.counts.were.calculated.with.background.rejection..Figure.16.9e.shows.the.
percentage.improvement.from.the.mean.photon.counts.according.to.the.imaging.depth..he.percentage.
improvement.shows.that.at.greater.imaging.depths,.the.improvement.becomes.larger,.so.the.improve-
ment.for.90-100%.intensity.pixels.was.38.1%.at.80.μm.depth..Figure.16.9f.shows.the.increment.to.the.
mean.photon.count..In.terms.of.photon-count.increment,.the.result.at.40.μm.depth.shows.the.greatest.
improvement. when. compared. to. counts. at. greater. depths.. At. shallower. depths,. the. compensation. is.
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FIGuRE 16.9 (See color insert.)
Signal.improvement.ater.AO.compensation..(a-d).Histograms.for.the..number.
of. pixels. according. to. their. intensity;. the.
x-
axis. represents. intensity. of. the. pixels.. For. example,. 10. means. that.
the. pixels. have. 0-10%. intensity. of. the. maximum. in. the. whole. image,. and. 100. means. 90-100%. intensity. pixels..
he.
y-
axis. represents. the. number. of. pixels. in. the. intensity. range.. he. let-placed. columns. show. the. number. of.
.pixels.before.the compensation,.and.the.right-placed.columns.show.the.result.ater.compensation..Each..histogram.
was. normalized. to. itself.. he. distribution. at. (a). 20. μm,. (b) 40. μm,. (c). 60. μm,. and. (d). 80. μm. imaging. depth..
(e) Percentage improvement.according.to.imaging.depth..he.curve.with.square.markers.shows.the.improvement.
with.background.rejection.(only.the.luorescent.area.was.calculated),.and.the.curve.with.triangular.markers.shows.
the.improvement.
