Biomedical Engineering Reference
In-Depth Information
on.the.same.physical.principles.as.CGWS..Within.its.operating.regime,.CGWS.is.expected.to.provide.a.
superior.wavefront.sensing.technique.for.weakly.stained.luorescent.samples,.where.bleaching.during.
image-based.wavefront.optimization.is.prohibitive..CGWS.relies.only.on.backscattering.of.the.excita-
tion.light,.so.it.is.independent.of.the.imaging.modality.it.is.combined.with.
Further.research.is.necessary.to.evaluate.the.possibilities.to.improve.measurement.depth.in.highly.
scattering.samples..For.example,.polarization-sensitive.detection,.confocal.iltering.of.the.backscattered.
light,.and.the.use.of.coherence.lengths.as.short.as.the.NA-determined.optical.section.thickness.could.all.
contribute.to.an.increased.measurement.depth.
Acknowledgments
We.thank.Sylvain.Gigan,.Jean-François.Léger,.Winfried.Denk,.and,.in.particular,.Laurent.Bourdieu.for.
discussions.and.comments.on.the.chapter.
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