Biomedical Engineering Reference
In-Depth Information
(a)
X
-
Y
X
-
Y
(b)
27,000
20,250
Y
Water-immersion
objective
LA
13,500
X
6,750
SA
0
No AO
AO
AO
Water
Slide
(c)
(d)
LA
-
Z
X
-
Z
30,000
(g)
X (AO)
Y (AO)
LA (No AO)
SA (No AO)
20,000
10,000
0
0
1
2
3
(
µ
m)
30,000
(h)
X
-
Z (AO)
Y
-
Z (AO)
LA
-
Z (No AO)
SA
-
Z (No AO)
20,000
No AO
AO
(e)
(f)
SA
-
Z
Y
-
Z
10,000
0
0
1
2
3
4
5
6
7
8
9
10 11 12
(
µ
m)
(i)
1.6
1.2
0.8
0.4
No AO
AO
0
FIGuRE 13.7 (See color insert.)
.Correcting.the.system.aberration.improves.images.of.a.500.nm.luorescent.bead.
immersed.in.the.design.medium.of.water.(inset,.upper.right)..Lateral.and.axial.images.before.(a,.c,.e).and.ater.(b,.
d,.f).AO.correction..Z.denotes.the.axial.direction,.and.LA.and.SA.denote.the.long.and.short.axes,.respectively,.of.
the.bead.image.before.correction..(g).Intensity.proiles.along.lines.drawn.in.the.lateral.plane.(red.and.green.in.a.
and.b)..(h).Similar.proiles.along.lines.drawn.in.the.axial.planes.(red.and.green.lines.in.c-f)..(i).he.inal.corrective.
wavefront.for.system.aberration.when.using.a.Zeiss.20x,.1.0NA.objective,.in.units.of.wavelength.(λ.=.850.nm)..Scale.
bar:.2.μm..(Ji,.N.,.et.al.,.
Nat. Meth
.,.7,.2,.141-147,.2010.)
