Biomedical Engineering Reference
In-Depth Information
AO on
AO off
50
µ
m
FIGuRE 12.12
he.bone.cavities.imaged.with.AO.on/of.at.145.μm.below.the.surface.
AO on
AO off
50
µ
m
FIGuRE 12.13
he.bone.cavities.imaged.with.AO.on/of.at.184.μm.below.the.surface.
Average intensity vs. depth
120
Intensity with AO
correction at 184
Intensity with AO
correction at 145
100
80
Intensity with AO off
60
40
20
0
0
40
80
120
160
200
(surface)
Depth (
µ
m)
FIGuRE 12.14
he.average.intensity.plot.of.the.nonlinear.signal.at.diferent.depths.with.AO.on/of.
two.data.sets..It.is.also.notable.that.the.two.intensity.plots.with.AO.on.showed.weaker.intensity.at.shal-
low.depths.(e.g.,.0-120.μm)..his.is.because.the.DM.shapes.corrected.at.depths.of.145.and.184.μm.create.
optical.aberrations.in.the.shallower.depths.
If.the.images.taken.from.145.μm.below.the.surface.are.used.as.the.basis.for.quantiied.analysis,.the.
average.pixel.value.increases.to.36.74.with.AO.on.from.32.80.with.AO.of..However,.if.only.the.pixels.
that.are.larger.than.100.(gray.scale).are.counted.in.the.image.with.AO.on,.which.roughly.represents.the.
two-photon.excitation.signal.from.the.GFP,.the.average.pixel.value.jumps.to.130.50.with.AO.on.from.
72.12.with.AO.of..his.represents.an.81%.increment.in.terms.of.intensity.
