Biomedical Engineering Reference
In-Depth Information
1.025
1.02
1.015
1.01
1.005
1
0.995
0.99
0.985
0
20
40
50
80
100
120
140
160
1.045
Normalized
metric value
1.04
1.035
1.03
1.025
1.02
1.015
1.01
1.005
1
0.995
0
20
40
60
80
100
120
1.45
1.4
1.35
1.3
1.25
1.2
1.15
1.1
1.05
1
0
50
100
AO loop cycles
150
200
250
FIGuRE 12.6
he.measured.metric.as.a.function.of.AO.loops.based.on.three.control.bases..Top,.the.measured.
metric.as.a.function.of.AO.controller.based.on.individual.actuator.SPGD;.middle,.the.measured.metric.as.a.func-
tion. of. AO. controller. based. on. grouped. actuator. SPGD;. and. bottom,. the. measured. metric. as. a. function. of. AO.
controller.based.on.Zernike.polynomial.SPGD.
In. the. focal. plane. of. the. illumination. laser. in. the. two-photon. scanning. laser. luorescence. micro-
scope.(TPSL-FM),.the.luorescent.intensity.is.highly.dependent.on.the.average.illumination.intensity.in.
the.lateral.plane.of.the.focal.volume..he.average.illumination.intensity.decreases.with.an.increase.in.
the width.of.the.point.spread.function.(PSF)..his.is.especially.signiicant.for.the.two-photon.luores-
cence.excitation,.due.to.the.nonlinear.nature.of.the.excitation.
Stronger. luorescence. intensity. can. be. generated. from. the. focal. plane. in. two-photon. luorescence.
excitation.in.the.absence.of.aberrations.[30]..he.generated.luorescence.intensity.can.be.simpliied.as
PSF
ρ
.
(
)
∫
.
F z W z
( )
=
2
( )
2
,
z d
2
.(12.1)
where.
W
(
z
).is.the.total.illumination.power.in.the.focal.plane;.PSF.is.the.point.spread.function.in.the.
focal.plane.[30];.ρ
.is.the.vector.corresponding.to.the.radial.coordinates.in.a.plane.perpendicular.to.the.
beam.axis;.and.
z
.is.the.coordinate.aligned.with.the.beam.axis,.which.is.deined.as.0.at.the.sample.surface.
