AO Two-Photon Fluorescence Microscopy Using Stochastic Parallel Descent Algorithm with Zernike Polynomial Basis - Adaptive Optics for Biological Imaging - page 209

12
AO Two-Photon
Fluorescence Microscopy
Using Stochastic Parallel
Descent Algorithm with
Zernike Polynomial Basis

12.1. Introduction.........................................................................................209
Advantages.of.Two-Photon.Fluorescence.Microscopy.in .
Tissue.Imaging. •. Two-Photon.Fluorescence.Excitation.and.
Emission. •. Optical.Resolution.and.Tissue.Optical.Aberrations
12.2. Background..........................................................................................212
12.3. Method..................................................................................................213
Stochastic.Parallel.Gradient.Descent.Algorithm.with.Zernike .
Polynomial.Basis. •. Metric.Selection.for.Stochastic.Parallel.Gradient .
Descent.Algorithm. •. Application.of.Stochastic.Parallel.Gradient .
Decent.Algorithm.with.Zernike.Polynomial.Basis
12.4. Optical.System.Design........................................................................220
12.5. Experiments.and.Results....................................................................222
Point.Spread.Function.Quantiication. •. Mouse.Bone-Marrow.Imaging .
with.Adaptive.Optics
12.6. Critical.Discussion..............................................................................226
12.7. Summary..............................................................................................227

Yaopeng Zhou
Abbott Laboratories

12.1 introduction

he.two-photon.luorescence.microscope.(TPFM).provides.a.powerful.tool.for.deep-tissue.imaging..Its.
unique.ability.of.seeing.through.the.tissue.surface.allows.researchers.to.acquire.high-resolution.images.
of.cell-level.details.up.to.a.few.millimeters.below.the.tissue.surface..Various.biological.tissues.such.as.
brain.[1],.heart.[2],.kidney.[3],.and.skin.[4].have.been.studied.by.imaging.with.TPFMs..he.TPFM.with.
adaptive.optics.(AO).described.in.this.chapter.applies.to.the.study.of.stem.cells.and.microenvironment.
in.the.bone.marrow.of.mouse.skull.bone..he.constructed.AO.TPFM.is.a.collaboration.efort.between.
Boston.University.(BU).and.Wellman.Center.for.Photomedicine.
Hematopoietic.stem.cells.(HSCs).reside.in.bone.marrow.and.are.responsible.for.the.maintenance.of.
all.cells.found.in.blood.(white.blood.cells,.red.blood.cells,.and.platelets).and.the.immune.system.(B.cells,.
T cells,.natural.killer.cells,.and.dendritic.cells).[5]..HSCs.and.their.clinical.applications.have.been.widely.
studied.[6,.7].

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