Biomedical Engineering Reference
In-Depth Information
be.introduced.via.the.sotware,.and.the.wavefront.deviations.can.be.compared.to.the.new.user-deined.
reference..For.a.research.group.that.is.just.starting.out.and.is.not.ailiated.with.an.AO.astronomy.or.
vision.science.group.that.has.already.developed.this.sotware,.this.is.a.way.to.get.of.to.a.quick.start.in.
biological.imaging.
Imagine.optics.ofers.a.MicAO.system,.an.add-on.for.commercial.biological.microscopes,.including.
super-resolution.approaches,.such.as.stochastic.optical.reconstruction.microscopy.and.photoactivated.
localization. microscopy.. A.schematic.diagram. of.the.optical.system.is.shown. in.
Figure.8.42
..he.AO.
system.is.designed.to.be.plugged.into.the.camera.port.of.a.microscope.to.correct.for.the.wavefront.aber-
rations.introduced.by.the.system..It.is.compatible.with.most.commercial.microscopes,.and.the.design.of.
the.system.allows.working.with.almost.all.the.commercial.biological.objectives.that.are.available..It.uses.
a.mirao™.52-d.electromagnetic.DM.and.a.sensorless.correction.algorithm,.as.described.in.Chapter 10,.
to.improve.the.image.quality..Since.the.system.does.not.require.a.wavefront.sensor,.the.cost.can.be.sub-
stantially.lower.than.an.AO.system.that.does.use.a.wavefront.sensor.
references
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