Biomedical Engineering Reference
In-Depth Information
Mode 2 - tip
Mode 3 - tilt
Mode 4 - defocus
(b)
1
0.5
0
̶
0.5
3
2
1
0
̶
1
̶
2
̶
3
̶
1
Mode 5 - astigmatism
Mode 6 - astigmatism
Mode 7 - coma
Mode 8 - coma
Mode 9
Mode 10
Mode 11 - spherical (1st)
Mode 12
Mode 13
Mode 14
Mode 2 - tip
Mode 3 - tilt
Mode 4 - defocus
(c)
4
2
2
1
0
0
̶
2
̶
4
̶
1
̶
2
Mode 5 - astigmatism
Mode 6 - astigmatism
Mode 7 - coma
Mode 8 - coma
Mode 9
Mode 10
Mode 11 - spherical (1st)
Mode 12
Mode 13
Mode 14
FIGuRE 6.3
(
Continued
)
his.simulated.sphere.could.be.regarded.as.a.very.simple.model.for.a.spherical.cell.within.a.water-
based.sample..he.model.sphere.had.a.refractive.index.of.1.35.and.cell.cytoplasm.has.typically.a.refrac-
tive.index.in.the.range.of.1.35-1.38.(Dunn.1998).
6.3 Modeling of an oocyte cell and comparison
to experimental results
he.aberrations.caused.by.a.mouse.oocyte.cell.are.modeled.to.verify.the.experimental.results.obtained.
by.interferometric.wavefront.measurements.
Since.this.particular.type.of.cell.has.a.basic.shape,.wavefront.simulations.can.be.based.on.a.simple.model..
he.transmitted.light.image.of.the.cell.is.shown.in.
Figure.6.4
.
next.to.the.model.used.for.the.simulations..he.
