Biomedical Engineering Reference
In-Depth Information
example- pGEM
®
-T
Figure9.2
Cloningvector
x-gal, a colourless liquid, to produce free galactose and 'x' which results in a blue
pigment to the colony. Thus the screening for successful insertion into the MCS
is a simple scoring of blue (negative) or white (possibly positive) colonies. The
success of the experiment can be determined quickly as this cloning vector also
has sequences at either side of the MCS which allows for rapid DNA sequencing.
Additionally, some eukaryotic viruses may be used as vectors but these tend
to be so large that direct cloning into them is difficult. A solution to this is to
carry out manipulations on the desired DNA fragment cloned into a bacterial
plasmid and then transfer the engineered piece into the virus thus making a
recombinant eukaryotic virus. One such virus now used extensively in GE, both as
a cloning vehicle and as an excellent expression vector, is Baculovirus illustrated
in Figure 9.3 which is also effective as a bioinsecticide.
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