Analytical Methods for Corona Evaluations - Protein-Nanoparticle Interactions - page 79

Fig. 4.6 ( a ) DCS data for SiO 2 nanoparticles incubated for 1 h in plasma solution at different
concentrations showing the effect of biological environment concentration on hard corona shell
thickness. ( b ) Corona shell thickness calculated using a model (Adapted from [ 17 ])

how the NTA technique can be extended to multiparameter analysis, allowing for
characterization of particle size and light-scattering intensity on an individual basis.
This multiparameter measurement capability allows subpopulations of nanoparticles
with varying characteristics to be resolved in a complex mixture. Changes in one or
more of such properties can be followed both in real time and in situ.
As it can be seen in Fig. 4.6 , DCS shows that the increase of nanoparticle
concentration in plasma solution or the dilution of the plasma solution changes
the thickness of hard corona which was discussed in detail in Chap. 2 .

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