Biomedical Engineering Reference
In-Depth Information
Raman
spectroscopy
Fluorescence and Rayleigh
scattering noise
X-ray
crystallography
(XRC)
3D structure of
NP-protein
complex
Need of crystallized objects
Nuclear Magnetic
Resonance
(NMR)
NP-bound protein
binding site
mapping
Line broadening in spectrum
when proteins bind to NP
Isolation and
separation of
NP-bound
proteins
Chromatography
Quantitative
Limited application range
Capillary
electrophoresis
(CE)
Quantitative
Limited detection sensitivity
One-dimensional
electrophoresis
(1D-E)
Simple, fast
Less effective separation
Two-dimensional
electrophoresis
(2D-E)
High ability of
separating proteins
More complex, less
repeatable
Identification of
NP-bound
proteins
Mass spectroscopy
(MS)
Efficient, low amount
of protein samples
Less quantitative
N-terminal
microsequencing
Direct determination of
amino acid
sequences of
proteins
Less quantitative
Kinetics
Quartz crystal
microbalance
(QCM)
Real time, label-free,
sensitive,
quantitative
Immobilization of one
component
Surface plasmon
resonance
spectroscopy
(SPR)
Real time, label-free,
sensitive,
quantitative
Immobilization of one
component
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