Biomedical Engineering Reference
In-Depth Information
Table 3.1
Description of the cell lines used in MTT and XTT studies (Adapted from [
16
])
Cell code
Cell type
Culture medium
BE(2)-C
Human neuroblastoma
1:1 (DMEM + Ham's F12) + FBS10 %
A172
Human glioblastoma
DMEM + FBS10 %
HCM
Human cardiac
myocytes
1:1 (DMEM + Ham's F12) + FBS10 %
supplemented with 5
μ
g/ml insulin
and 50 ng/ml bFGF
A549
Human lung
adenocarcinoma
DMEM + FBS10 %
Hep G2
Human hepatocellular
carcinoma
RPMI 1640 + FBS 10 %
A-431
Human epithelial
carcinoma
DMEM + FBS 10 %
293T
Human embryonic
kidney
RPMI 1640 + FBS10 %
SW480
Human colon
adenocarcinoma
DMEM + FBS10 %
HeLa
Human cervical
adenocarcinoma
MEM + FBS10 %
Capan-2
Human pancreas
adenocarcinoma
RPMI + FBS10 %
Panc-1
Human pancreatic
carcinoma
DMEM + FBS10 %
Jurkat
Human T cell
lymphoblast like
RPMI + FBS10 %
L929
Mouse connective
tissue fibroblast
RPMI + FBS10 %
Several studies using ex vivo perfusion have shown that the liver uptake of lecithin
polystyrene nanoparticles, PMMA nanoparticles, and liposomes is reduced by
preincubation with serum. Nanoparticles carry repetitive chemical patterns such
as ligands and chemical groups on the surface. Macrophage scavenger receptors
(SRs) are a broad group of phagocytic receptors that are responsible for the
elimination of blood-borne viruses, pathogens, and negatively charged ligands.
Several reports have shown that polyanionic ligands of scavenger receptors, includ-
ing polyinosinic acid, fucoidan, and dextran sulfate, could inhibit the uptake of
quantum dots [
15
], carbon nanotubes, iron oxide, and polystyrene.
As complementary factor to the protein corona, there is a crucial matter that
should be greatly considered for the safe design of any type of nanoparticles, which
is called the cell “vision” [
16
,
17
]. Cell vision is recognized as the numerous
detoxification strategies that any particular cell can utilize in response to
nanoparticles. The defense mechanism could be considerably different according
to the cell types. Thus, what the cell “sees,” when it is faced with nanoparticles, is
most likely dependent on the cell type. For example, various cellular (see Table
3.1
)
responses (e.g., uptake and reactive oxygen species (ROS) production) to the exact
