Biomedical Engineering Reference
In-Depth Information
With this geometry, SHG light generated in the specimen travels back along the laser light path, pass-
ing through the scanning system before being decoupled from the laser path. In this way, the focus of
SHG light detected by the PMT always occupies the same position (as in confocal microscopy), allowing
the use of a detector with a very small sensitive area.
In this configuration, the distance between the detector and the collection objective drastically
reduces the collection of multiple-scattered SHG photons, limiting
in vivo
applicability.
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