Biomedical Engineering Reference
In-Depth Information
Specimen
L4
BF
DM
PMT
BP
TL
λ
/2
Acquisition
PBS
S
L3
Control
G
x
G
y
L1
L2
FIgurE 2.18
Optical scheme of a typical SHG microscope with backward detection geometry (non-de-scan
mode). Ti:sapphire:femtosecond laser source; λ/2: rotating half-wave plate; PBS, polarizing beam splitter cube;
S, shutter;
G
x
, galvo-scanner
x
; L1, relay lens; L2, relay lens;
G
y
, galvo-scanner
y
; L3, scanning lens; TL, tube
lens; DM, dichroic mirror; BF, laser-blocking filter; L4, collection lens; BP, narrow band-pass filter; PMT, photo-
multiplier detector; POL, polarizer; λ/4, quarter-wave plate; RP, rotating polarizer; Exc, excitation/collection
objective lens.
Specimen
λ
/2
PBS
TL
S
BP
Acquisition
DM
PMT
L4
BF
L3
G
x
G
y
Contro
l
L1
L2
FIgurE 2.19
Optical scheme of a typical SHG microscope with backward detection geometry (de-scan mode).
Ti:sapphire:femtosecond laser source; λ/2: rotating half-wave plate; PBS, polarizing beam splitter cube; S, shut-
ter; DM, dichroic mirror; BF, laser-blocking filter; L4, collection lens; BP, narrow band-pass filter; PMT, photo-
multiplier detector;
G
x
, galvo-scanner
x
; L1, relay lens; L2, relay lens;
G
y
, galvo-scanner
y
; L3, scanning lens; TL,
tube lens; POL, polarizer; λ/4, quarter-wave plate; RP, rotating polarizer; Exc, excitation/collection objective lens.
