Biomedical Engineering Reference
In-Depth Information
(a)
(d)
(g)
(e)
(b)
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(f )
(i)
70
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10
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SHG
Picrosirius red
SHG
Picrosirius red
SHG
Picrosirius red
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1
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1
Figure 16.6
Highlighted fiber orientation relative to the horizontal was measured in Gimp, a freely available
imaging processing tool. The graph shows average angle orientation with error bars indicating (+/−) standard devi-
ations of the 5 angle measurements per sample of both the SHG and Picrosirius techniques. Scale bars = 10 μm.
200
180
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Figure 16.11
Use of CurveAlign to quantify collagen orientation in a human breast cancer specimen. An SHG
image of a histopathological section (a) was analyzed by CurveAlign (b) and exported as a histogram (c).
