Biomedical Engineering Reference
In-Depth Information
4°C
14°C
24°C
37°C
(a)
(b)
(c)
(d)
50 μm
(e)
(f )
(g)
(h)
500 nm
pH 5.5
pH 6.5
pH 7.5
pH 8.5
(i)
(j)
(k)
(l)
50 μm
(m)
(n)
(o)
(p)
500 nm
Figure 11.1
Simultaneously collected SHG (blue signal) and TPF (green signal) images of 4 mg/mL acellular
collagen hydrogels polymerized at various temperatures (a-d) and pH values (i-l), with corresponding SEM
images (e-h, m-p). The scale bars are indicated in the figure. (Reprinted from
Biophys J
., 92, Raub, C. B. et al.,
Noninvasive assessment of collagen gel microstructure and mechanics using multiphoton microscopy, 2212-
2222, Copyright 2007;
Biophys J
., 94, Raub, C. B. et al., Image correlation spectroscopy of multiphoton images
correlates with collagen mechanical properties, 2361-2373, Copyright 2008, with permission from Elsevier.)
(a)
Fiber diameter
Mesh size
Figure 11.8
(a) Combined SHG + TPF (SHG, blue signal; TPF, green signal) image of acellular collagen hydro-
gel showing examples of measurements of collagen fiber diameter and network mesh size. (Reprinted from
Biophys
J
., 94, Raub, C. B. et al., Image correlation spectroscopy of multiphoton images correlates with collagen mechanical
properties, 2361-2373, Copyright 2008, with permission from Elsevier.)
