SHG Imaging for Tissue Engineering Applications - Second Harmonic Generation Imaging - page 423

matrix and a widely used scaffold material. In addition, two other promising scaffold materials are
SHG-active, namely biosynthesized cellulose and silk fibroin. Finally, one of the major components of
skeletal muscle tissue, myosin, can also be selectively visualized using SHG microscopy. These imaging
abilities are sufficient to provide relevant information on a multitude of tissue engineering constructs,
as confirmed by the increasing number of publications where SHG microscopy has been applied for this
purpose. This growing trend can be expected to continue since recent developments in laser technology
promote the spread and future establishment of the SHG technique within tissue engineering as well as
other fields of chemistry and biology.
Acknowledgments
The authors would like to gratefully acknowledge the following persons for their contributions to this
chapter; Mr. Matthew Dalene and Associate Professor Jan Stegemann, University of Michigan (Figure
18.2); Ms. Ana-Maria Pena, ĹOréal Research and Innovation (Figure 18.3); Mr. Willam Rice and
Associate Professor Irene Georgakoudi, Tufts University (Figure 18.11). The long-term fruitful collabora-
tion with the group of Professor Paul Gatenholm, Polymer Science, Chalmers University of Technology,
Sweden, on the studies of biosynthesized cellulose is gratefully acknowledged by the authors.
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