Biomedical Engineering Reference
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(a)
(b)
800 nm
1064 nm
60
45
30
15
0
60
45
30
15
0
20 μm
20 μm
(c)
1.31% w/w
(d)
2.25% w/w
15
10
5
0
15
10
5
0
20 μm
20 μm
(f )
8
(e)
3.76% w/w
6
4
2
15
10
5
0
0
0
1 2 3
Cellulose density (% w/w )
4
5
20 μm
(g)
(h)
20 μm
20 μm
(i)
(j)
150
100
50
0
150
100
50
0
5 μm
5 μm
FIgurE 18.6 SHG microscopy data obtained from microbial biosynthesized cellulose. Comparing excitation
at 800 nm (a) and 1064 nm (b) shows similar signal strengths for both cases. SHG images measured at different
sample densities expressed as % w/w cellulose; (c) 1.31% w/w , (d) 2.25% w/w , and (e) 3.76% w/w . (f ) Average SHG-signal
versus sample density, an increase in epi-detected SHG signal is obtained with increasing sample density, due to
more efficient back-scattering of the signal. SHG images measured in the same fields of view using vertical and
horizontal laser polarizations (double arrows). (g) and (h) 100 × 100 μm images, (i) and (j) 40 × 40 μm images. The
large-scale material structures appear similar, (g) and (h), however, fibers aligned with the laser polarization give
the dominant signal contribution, (i) and (j).
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