Biomedical Engineering Reference
In-Depth Information
From this additional elegant model, we can also infer that if a sufficient proportion of collagen triple
helixes are not axially aligned with the fibril axis, this may impact our ability to obtain accurate helical
pitch angle measurements as per Model 1 above, and this may be another factor to account for some of
the small discrepancies seen between SHG-based and x-ray diffraction-based measurements of collagen
helix pitch angle.
Overall, we see that SHG intensity under varied polarization states contains not just information as
regards the orientation of the collagen fibril in the
xy
plane, but we can also extract information regard-
ing the molecular structure of collagen, namely, collagen's helical pitch angle and the angular orienta-
tion of collagen triple helices relative to the fibril axis.
The equations and text in this box are reprinted (with some modifications) with permission from
Han, X. et al. 2008. Second harmonic properties of tumor collagen: Determining the structural relation-
ship between reactive stroma and healthy stroma.
Opt Express
,
16
:1846-1859. Copyright 2008, Optical
Society of America. The figures in this box are reprinted with permission, as indicated.
References
1. Han, X., R.M. Burke, M.L. Zettel, P. Tang, and E.B. Brown. 2008. Second harmonic properties
of tumor collagen: Determining the structural relationship between reactive stroma and healthy
stroma.
Opt Express
,
16
:1846-1859.
2. Kalluri, R. and M. Zeisberg. 2006. Fibroblasts in cancer.
Nat Rev Cancer
,
6
:392-401.
3. Shekhar, M.P., R. Pauley, and G. Heppner. 2003. Host microenvironment in breast cancer develop-
ment: Extracellular matrix-stromal cell contribution to neoplastic phenotype of epithelial cells in
the breast.
Breast Cancer Res
,
5
:130-135.
4. Haslam, S.Z. and T.L. Woodward. 2003. Host microenvironment in breast cancer development:
Epithelial-cell-stromal-cell interactions and steroid hormone action in normal and cancerous
mammary gland.
Breast Cancer Res
,
5
:208-215.
5. Hasebe, T., S. Sasaki, S. Imoto, K. Mukai, T. Yokose, and A. Ochiai. 2002. Prognostic significance
of fibrotic focus in invasive ductal carcinoma of the breast: A prospective observational study.
Mod
Pathol
,
15
:502-516.
6. Jukkola, A., R. Bloigu, K. Holli, H. Joensuu, R. Valavaara, J. Risteli, and G. Blanco. 2001. Postoperative
PINP in serum reflects metastatic potential and poor survival in node-positive breast cancer.
Anticancer Res
,
21
:2873-2876.
7. Jensen, B.V., J.S. Johansen, T. Skovsgaard, J. Brandt, and B. Teisner. 2002. Extracellular matrix build-
ing marked by the N-terminal propeptide of procollagen type I reflect aggressiveness of recurrent
breast cancer.
Int J Cancer
,
98
:582-589.
8. Keskikuru, R., R. Bloigu, J. Risteli, V. Kataja, and A. Jukkola. 2002. Elevated preoperative serum ICTP
is a prognostic factor for overall and disease-free survival in breast cancer.
Oncol Rep
,
9
:1323-1327.
9. Brown, E., T. McKee, E. diTomaso, A. Pluen, B. Seed, Y. Boucher, and R.K. Jain. 2003. Dynamic
imaging of collagen and its modulation in tumors
in vivo
using second-harmonic generation.
Nat
Med
,
9
:796-800.
10. Perentes, J.Y., T.D. McKee, C.D. Ley, H. Mathiew, M. Dawson, T.P. Padera, L.L. Munn, R.K. Jain,
and Y. Boucher. 2009.
In vivo
imaging of extracellular matrix remodeling by tumor-associated fibro-
blasts.
Nat Methods
,
6
:143-145.
11. Hompland, T., A. Erikson, M. Lindgren, T. Lindmo, and C. de Lange Davies. 2008. Second-harmonic
generation in collagen as a potential cancer diagnostic parameter.
J Biomed Opt
,
13
:054050.
12. Zipfel, W.R., R.M. Williams, R. Christie, A.Y. Nikitin, B.T. Hyman, and W.W. Webb. 2003. Live tissue
intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic
generation.
Proc Nat Acad Sci USA
,
100
:7075-7080.
13. Wilder-Smith, P., T. Krasieva, W.G. Jung, J. Zhang, Z. Chen, K. Osann, and B. Tromberg. 2005.
Noninvasive imaging of oral premalignancy and malignancy.
J Biomed Opt
,
10
:051601.
