Biomedical Engineering Reference
In-Depth Information
FIgurE 15.7
SHG imaging of human liver fibrosis. Comparison between SHG imaging (top) and transmitted-
light microscopy after Sirius red staining (bottom) of human liver fibrosis (F3-Metavir biopsy). Right pictures show
high-magnification imaging performed in the area delimited by the dotted square. Samples were 5 μm thick. Laser
excitation: 100 mW at 810 nm wavelength; scale bar: 1 mm. (Reprinted from
J. Hepatol
., 52, Gailhouste, L. et al.,
Fibrillar collagen scoring by second harmonic microscopy: A new tool in the assessment of liver fibrosis, 398-406,
Copyright 2010, with permission from Elsevier.)
FIgurE 15.8
Simultaneous imaging of TG2 activity and fibrillar collagen deposition. SHG imaging (light grey)
reveals collagen fibrosis and 2PEF from His
6
-Xpress-GFP labeling (dark grey) highlights TG2 activity in the kid-
ney cortex of a fibrotic mice (28-day AngII infusion). Laser excitation: 20 mW at 800 nm. Scale bar: 50 μm. (From
Strupler, M. et al., 2008.
J. Biomed. Optics
13:054041. With permission of SPIE.)
to be a good substrate for tissular transglutaminase (TG2), an enzyme that catalyzes crosslinking of
collagen molecules within fibrils (Furutani et al. 2001). Combined SHG/2PEF imaging then enables
the compared localization of the TG2 activity and of the fibrosis extent within the kidney cortex.
15.3.2.4 Fibrosis/inflammation
An interesting application of the multimodal approach described above concerns the simultaneous
visualization of fibrosis and inflammation by use of combined SHG/2PEF microscopy of unstained
