Biomedical Engineering Reference
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(a)
(b)
(c)
80 μm
80 μm
80 μm
LC
IE
L
L
CARS
SFG
TPEF
(d)
(e)
(f )
300 μm
300 μm
300 μm
LC
LC
L
L
(g)
(h)
(i)
30 μm
30 μm
30 μm
Dox. labeled
FIgurE 13.10 ( See color insert .) Representative images of a Type IV lesion inspected by CARS (a), SFG (b), and
TPEF (c). The lipid core (LC) was identified by CARS. (d and e) Masson's trichrome staining. Black rectangle: the
corresponding area in (a) through (c). (f) H&E staining. (g and h) Colocalized NLO images corresponding to the
red and yellow squares in (a), respectively. (i) Doxorubicin-labeled (red) image, around lipid core, colocalized with
CARS and SFG signals. (Reproduced from Wang, H. W. et al. 2009. Arterioscl Throm Vas 29:1342-1348. With the
permission of SPIE.)
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